ABTS SUBSTRATE

ABTS SUBSTRATE - Names and Identifiers

Name	2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid Ammonium Salt
Synonyms	ABTS ABTS-(NH4)2 ABTS SUBSTRATE ABTS DIAMMONIUM SALT ABTS(R), DIAMMONIUM SALT ABTS(TM) CHROMOPHORE, DIAMMONIUM SALT Diammonium 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonate) 2,2'-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid Ammonium Salt 2,2'-AZINOBIS(3-ETHYLBENZOTHIAZOLINE-6-SULFONIC ACID AMMONIUM SALT) 2,2'-AZINO-BIS(3-ETHYLBENZOTHIAZOLINE-6-SULFONIC ACID) DIAMMONIUM SALT Diammonium 2,2'-azinobis(3-ethyl-2,3-dihydrobenzothiazole-6-sulphonate) 2,2'-Azino-di(3-ethyl-benzthiazoline sulphonic acid (6)), ammonium salt 6-Benzothiazolesulfonic acid, 2,2'-azinobis(3-ethyl-2,3-dihydro-, diammonium salt diammonium (2Z)-3-ethyl-2-[(2E)-(3-ethyl-6-sulfonato-1,3-benzothiazol-2(3H)-ylidene)hydrazono]-2,3-dihydro-1,3-benzothiazole-6-sulfonate
CAS	30931-67-0
EINECS	250-396-6
InChI	InChI=1/C18H18N4O6S4.2H3N/c1-3-21-13-7-5-11(31(23,24)25)9-15(13)29-17(21)19-20-18-22(4-2)14-8-6-12(32(26,27)28)10-16(14)30-18;;/h5-10H,3-4H2,1-2H3,(H,23,24,25)(H,26,27,28);2*1H3/b19-17-,20-18+;

ABTS SUBSTRATE - Physico-chemical Properties

Molecular Formula	C18H24N6O6S4
Molar Mass	548.68
Melting Point	>181oC (dec.)
Water Solubility	Dissolve in water at 50mg/ml. Solution may be slightly hazy.
Solubility	Dissolve in water at 50mg/ml. Solution may be slightly hazy.
Appearance	tablet
Color	Pale Green to Light Green
BRN	7329461
PH	pH(50g/l, 25℃) : 5.0～6.0
Storage Condition	2-8°C
Stability	Hygroscopic, Light Sensitive
Sensitive	Light Sensitive
MDL	MFCD00010404
Use	This product is for scientific research only and shall not be used for other purposes.
In vitro study	A micro-technique of enzyme-linked immunosorbent assay (ELISA) using ABTS, as a substrate for HRP conjugate is studied. In a comparative study among 4 substrates, namely; 5-aminosalicylic acid (5AS), O-phenylenediamine (OPD), O-tolidine (OT) and ABTS, for HRP in terms of sensitivity, ABTS is the most sensitive, stable and the best in visuality by its bluish-green color. ABTS is a typical peroxidase substrate. For purification and characterization peroxidase positive transformants are cultivated in large scale (XL) under conditions that yield active protein in the culture supernatant. After 160 h cultivation an activity of 55,000 U/L in relation to the substrate ABTS is achieved and the supernatant containing the peroxidase is harvested. With ABTS as substrate the peroxidase activity falls significantly when the H 2 O 2 concentration rose above 0.125 mM, indicating that the enzyme is inhibited by H 2 O 2 . Maximum reaction rates depending upon substrate tested reache values between 31.2 and 125 µM.

ABTS SUBSTRATE - Risk and Safety

Hazard Symbols	Xi - Irritant
Risk Codes	36/37/38 - Irritating to eyes, respiratory system and skin.
Safety Description	S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing.
WGK Germany	3
RTECS	DL7002000
FLUKA BRAND F CODES	8-10-23
HS Code	29342080

ABTS SUBSTRATE - Introduction

A spectroscopic reagent for free chlorine, an enzyme immunochromogenic substrate, a substrate for peroxidase, is used in the ELISA step, which produces a soluble green end product that can be spectral read at 405 nm. This reaction can be terminated with 1% SDS.

Last Update：2022-10-16 17:24:29

ABTS SUBSTRATE - Reference Information

biological activity	ABTS diammonium salt is a substrate for horseradish peroxidase (HRP).
Use	A peroxidase matrix suitable for the ELISA step. This matrix produces a soluble green end product and can be used in a spectrophotometer Observed at 405nm; free chlorine spectroscopic reagent, enzyme-free chromogenic substrate peroxidase substrate free chlorine spectroscopic reagent, enzyme-free chromogenic substrate, peroxidase substrate, for the ELISA step, the substrate produces a soluble green end product that can be spectral read at 405 nm. This reaction can be terminated with 1% SDS.
category	toxic substances
flammability hazard characteristics	combustible; combustion produces toxic nitrogen oxides and sulfur oxide smoke
storage and transportation characteristics	ventilation and low temperature drying
fire extinguishing agent	dry powder, foam, sand, carbon dioxide, mist water

Last Update：2024-04-09 02:00:08

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